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حمض BES العازل الحر (CAS 10191-18-1) هو عازل zwitterionic مع نطاق pH 6. 4- 7.8، مثالية للبحوث الكيميائية الحيوية.
Used in calcium phosphate-mediated DNA transfection for stable cell transformation.
Suitable for diagnostic assay manufacturing and protein studies due to its minimal interference.
Acts as a cross-linking agent in sulfonated polyimide membranes.
White crystalline powder with high solubility in water (1M at 20°C).
Melting point of 150-155°C and pKa of 7.1 at 25°C.
Low absorbance at 260 nm (≤0.05), making it suitable for UV-sensitive applications.
Compatible with Good's buffers, ensuring wide applicability in biological studies.

الأسئلة:

What is the pH range of BES Buffer Free Acid?

BES Buffer Free Acid has a useful pH range of 6.4 to 7.8, making it ideal for a variety of biochemical applications.
How is BES Buffer used in DNA transfection?

BES Buffer is used in calcium phosphate-mediated DNA transfection, where it facilitates the slow formation of calcium phosphate-DNA complexes, enhancing stable cell transformation efficiency.
Is BES Buffer suitable for protein studies?

Yes, BES Buffer is suitable for protein studies, though it may reduce color development in BCA protein assays by 30-35% at 50 mM due to potential Cu2+ binding.

10191-18-1 BES عازل الحمض الحر N,N-Bis ((2-Hydroxyethyl)-2-Aminoethanesulfonic Acid